All bacterial species can be produce enzyme?

Enzyme is a protein molecule and are biological catalysts , Microorganisms are the primary source of enzymes, because they are cultured in large quantities in short span of time and genetic...

02 March 2019 2,847 4 View

Classification of bacteria depending of oxygen requirement ?

The environments in which some prokaryotes grow would kill most other organisms, the major physical factors which affect microbial growth are solutes and water activity, pH, temperature, oxygen...

31 December 2018 6,159 0 View

Problem in glucoamylase purification by gel filtration technique ?

31 December 2018 4,557 1 View

Show the type of culture media can be used for indole production test?

This test determines whether the microbe produces indole from the amino acid tryptophan. If indole is produced, it will react with a chemical reagent added after incubation to produce a color change.

31 December 2018 1,938 0 View

Determination of the glucose oxidase kinetic parameters (Km and Vmax) assay ?

The reaction rate of glucose oxidase was determined at different glucose concentration ( 20 , 12.5 , 10 , 5 , 3.33 , 2.5 ) % of glucose under optimum conditions of pH , temperature and incubation...

31 December 2018 6,532 1 View

Compare between the Azoles & Echinocandins ?

Azoles : Agents : ketoconazole, fluconazole, itraconazole, voriconazole, posaconazole, multiple topical formulations , The azoles are a broad class of antifungal agents , Echinocandins are a...

31 December 2018 4,813 1 View

Show the major nutritional types of procaryotes ?

All living organisms require a source of energy. Organisms that use radiant energy (light) are called phototrophs. Organisms that use (oxidize) an organic form of carbon are called heterotrophs or...

31 December 2018 5,300 0 View

Problem in the Optimization of the growth conditions for maximum production of the peroxidase by Bacillus subtilis?

This working was achived in Section of Biology,faculty of Science, Kufa university which contain peroxidase separation and classically of route parameters to work greatest defer of peroxidase...

31 December 2018 9,207 0 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View