Hello,
I also want to do some biochemical analyses and need to take some blood of the rats. To take the blood I need to use anesthetics. But I want to evaluate TNF-a and oxidative stress in the brain. Which anesthetic does no influence on this? I'm working on the model of VPA to induce austism.
Your Ethical Committee will force you to use anesthetics before this very painful procedure. As long as you mention that you give anesthesia for the rat it is not very important that it might affect the samples you take and the result you have. So, use what is suitable for you. Thiopentone intraperitoneally is a good choice.
Concious Sedation by the use of anaethetic drug will provide much safety . We shoud go
Quezia Damaris Jones Severino Vasconcelos .
Oi!
I think that it's actually essential to use an anesthetic because of the ethical concerns (you could be authorized to not use it if you can prove that it could interfere with the quality of your data). Normally, it's preferable to use an aesthetic cocktail to ovoid the single high dose monoanesthesia toxicity.
Here I found you a comparative study of different methods of animal euthanasia to collect brain samples for metabolic profiling.
Article Commonly Used Anesthesia/ Euthanasia Methods for Brain Colle...
Good luck,
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