The trouble with ion pairing agents is/are that they coat the column (stationary phase), thus permanently changing the column's selectivity (a C18 column is no longer a C18 column). Thus, once an ion pairing agent is used the column has to be dedicated for that particular separation.
I would avoid 'ion pairing agents' at ALL costs! If you are not getting RP-LC retention try a more hydrophobic phenyl phase and then try HILIC (if the molecule is particularly water loving).
The trouble with ion pairing agents is/are that they coat the column (stationary phase), thus permanently changing the column's selectivity (a C18 column is no longer a C18 column). Thus, once an ion pairing agent is used the column has to be dedicated for that particular separation.
Got the answer but TFA in small concentration is also used as ion pairing agent..its only 0.1% TFA and i want to use the same one octane sulphonic acid.