Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Can Microplastics act as vector of chemical transport into human system on the model of stomach oil extraction ?

Chemical transport from micro-plastics to animal tissues has been reported in birds.

01 August 2019 9,216 1 View

I need more article on the assessment of nutritional status in school age children?

01 July 2019 6,210 2 View

How do You determine the ranges of value for your parameters, time and temperature using soxhlet extraction(N-hexane And Ethanol) for lemon grassoil?

I'm trying to conduct an experiment on the optimization of extraction of lemongrass oil using soxhlet extraction. My Optimum parameters are temperature, time and lemongrass to solvent ratio. I'm...

08 April 2019 9,256 1 View

Can I pool sera of chickens within a replicte together for LH FSH ELISA analyses in order to reduce cost of analyses?

I need to run ELISA analyses for reproductive hormones (LH, FSH) in chickens. The samples are however too many. Can I pool sera of birds within a replicate/treatment together for the analyses in...

19 August 2018 9,819 2 View

How do I plot my data using the Sigmoidal Langmuir equation?

I've included an image with showing the equation. I need to know what parameters to plot on the x and y axes. Any help will be highly appreciated.

15 January 2018 290 3 View

Which basis set is the most laudable for studying reactions involving halides?

23 November 2014 5,142 2 View

How to identify the bands from amplification products which appear in gel electrophoresis and how to explain the origin of each of them?

Bands identification in PCR gel electrophoresis

23 May 2014 7,084 2 View

Green organic solvents

I'd like to know if there are any solvents that can be used for the extraction of organic compounds but is less hazardous than say Hexane. Thanks

11 November 2011 5,050 8 View

Is there a way to calculate iron speciation due to pH?

I'd like to be able to figure out what species of iron is predominant at any given pH.

29 September 2011 1,265 22 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View