To study a special protein I will enrich it by immunoprecipitation using antibody-beads. My question is "do I need to run a WB and cut the according size gel? or directly process the proteins on beads and digest them for MS?".
What are the pros and cons?
Divya Dhasmana
Hi Mingqi Li It is better to run the sample on SDS gel and stain with comassie to confirm the protein, Once you have confirmed it, you can go either way: either cut the band of interest from the gel or an on bead digestion. Please take into consideration the amount and the purity of protein required for your work. Good luck!
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