8 Questions 4 Answers 0 Followers
Questions related from Mingqi Li
Hello guys, when I calculate the synergetic effect in the matrix, I got 2 questions. 1st, is it necessary to be n=3? The following question is, if n should be ≥3, I will fill in the data with...
29 April 2025 6,341 0 View
When I orthotopically transplant KPC-LUC (single clone) into C57BL/6 mice to establish pancreatic ductal adenocarcinoma (PDAC) model, the luc signal is not consistent with the tumor size, or even...
08 January 2025 4,104 2 View
Hi everyone, I met a problem. I cannot see any signal of brca2. I just made a 4% SDS gel, and did transmembrane for 3 h under 300 mA, but there was no signal when I did ECL exposure. Because...
04 August 2023 4,363 2 View
CRISPR KO Cells need to do single clone, then we can acquire 100% KO. How about siRNA? Can we do transfection to get a KD pool, then do single clone to have the 100% KO?
10 April 2023 1,850 1 View
Please help me with the SDS-PAGE issue. I cannot see the protein of interest but the dirty dots all over the blots. And the dots are likely to gather around the pore of the gel holder cassette....
20 March 2023 6,787 2 View
To study a special protein I will enrich it by immunoprecipitation using antibody-beads. My question is "do I need to run a WB and cut the according size gel? or directly process the proteins on...
13 May 2022 4,606 1 View
Could you please list some common methods for identifying the binding pocket? I mean we have a compound and a protein of interest, so which domain of the protein will be binding with the compound?...
01 January 1970 5,305 5 View
Hello all, these days I'm working on a hard study (to me). I use 3C protease, flagellin, and poly dadt to induce NLRP1, NLRC4, and AIM2 inflammasome activation, respectively, to see the compounds'...
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