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Hi everyone, I met a problem. I cannot see any signal of brca2. I just made a 4% SDS gel, and did transmembrane for 3 h under 300 mA, but there was no signal when I did ECL exposure. Because...
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Please help me with the SDS-PAGE issue. I cannot see the protein of interest but the dirty dots all over the blots. And the dots are likely to gather around the pore of the gel holder cassette....
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Hello all, these days I'm working on a hard study (to me). I use 3C protease, flagellin, and poly dadt to induce NLRP1, NLRC4, and AIM2 inflammasome activation, respectively, to see the compounds'...
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