Dear ResearchGate community, hello!
I'm going to do an MTT test on glioblastoma cells with our drugs. I plan to add 10,000 cells in 100 µl medium with FBS per well to a 96-well plate, incubate for 24 hours and then add 50 µl of drugs. Incubate the cells with treatment for 24 hours and add MTT dye.
Tell me, please, should I remove the medium before adding the drugs (they are diluted in DMEM without FBS) and should I remove the drugs before adding MTT dye?
Thank you in advance!
Hello Sofya Landynya
Yes.
Before treating the cells, the medium must be removed so that the cells are exposed to a uniform treatment.
Before adding the MTT dye, it is necessary to remove the medium because when you remove the medium containing the drug, it ensures that there is no interference of the drug with the MTT reagent, and that all the cells are treated in the same manner during the MTT assay.
Best.
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