I wanted to perform RNA extraction of U937 cells (Human Myeloid Lymphoma) by TRIZOL method, after adding TRIZOL to the sample I had kept it for incubation at room temp. and since I was not able to Extract RNA immediately I thought that I will keep it at -80C however I forgot to keep the samples at -80 before leaving. I wanted to know If I should proceed with RNA extraction as It will 2 days that the sample will be in trizol and after which I can process it.
Thank you.
Hello Shreyas Gujar
You shouldn’t have forgotten to keep the sample at -80 degree C before leaving the lab. Anyway, such incidences keep happening! For your information, RNA is stable in trizol which deactivates RNases. However, the sample can be kept at room temperature at this point for, at most, a few hours, or the sample can be frozen for later use at -80°C.
So, you should not proceed with RNA extraction for this sample which has been kept at room temperature for two days.
Best.
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