I wish to make a drosophila feeding medium with rotenone final concentration of 500uM. I came to know the way it is dissolved and the solvents involved, but am not clear with the full protocol. Despite going through various papers, I couldn't find much. I request help in this at the earliest.
Thank you
There are two usually followed ways to feed a compound of interest to flies. Either using capillary tubes or directly by adding in a media. For you if first one works go with it. And if you want to add it to media, as rotenone is less soluble in water you can use an organic solvent such as DMSO. Dissolve your compound in a DMSO and have a stock solution. Then calculate the volume of stock required in your liquid media to make desired concentration (500uM). Add stock solution of compound (mix well) when media is bit cool before it solidifies.
Hope this will work for you.
Solubility of rotenone in DMSO is 0.5mg/ml only and maximum concentration of DMSO I can use on flies medium is not more than 0.5-2%. But according to the rotenone solubility in DMSO and required weight of rotenone to be used to make 500uM, the concentration of DMSO to be used in food is large which is lethal to flies. Is there any other way or alternative, please suggest.
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