Shreyas Gujar

3 Questions 2 Answers 0 Followers

Questions related from Shreyas Gujar

Standardization of Overlap PCR or 2-Step PCR and its Yield?

Hi fellow researchers... I am trying to construct an Overlap PCR product in order to ligate it but am having difficulties in standardizing the protocol. I am able to perform the individual PCR and...

22 November 2023 8,461 5 View

Should I proceed if I accidently over incubated the sample after adding trizol?

I wanted to perform RNA extraction of U937 cells (Human Myeloid Lymphoma) by TRIZOL method, after adding TRIZOL to the sample I had kept it for incubation at room temp. and since I was not able to...

18 February 2023 7,050 1 View

Should I proceed with RNA extraction by TRIZOL, if the sample in in trizol for 2 days.

I wanted to perform RNA extraction of U937 cells (Human Myeloid Lymphoma) by TRIZOL method, after adding TRIZOL to the sample I had kept it for incubation at room temp. and since I was not able to...

01 January 1970 1,350 3 View