Are we suppposed to include dead cells that have not proliferated when calculating in vitro proliferation of T cells. If we keep only live cells we could be in a situation where the majority of the cells have died and the 10 live cells are proliferating thus we would have 100% if we consider only the live cells.
Michael R. Hughes
There are probably exceptions but I would think that your in vitro assay would be invalid if you are getting the extreme case you are mentioning. You can remove dead cells from the % of CFSE-negative but ALSO report your % of dead cells. That would give evaluators of your data the most information. However, if you have high % dead cells - consider also adjusting parameters of your assay.
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