Hi,

I am trying to characterize some MRSA strains with PFGE. The DNA marker (SC DNA ladder or Lambda DNA ladder) migrates nicely through the agarose gel. The bacterial DNA on the other hand does not. I see the DNA stacked in the well. I am not sure whether the bacterial genomic DNA is digested or not. I tried several protocols but were not successful. Any recommendations? Should the genomic bacterial DNA migrate in the gel even if it is not digested?

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