Should Ellman reagent prepare freshly for every measurment? Would it be possible do Ellman assay by 96well plate ELISA reader or nanodrop?

Parastou Rahimizadeh @Parastou-Rahimizadeh

22 May 2024 1 4K Report

I want to measure the amount of free Cys after reduction by DTT by Ellman assay. is there any possibility of measuring absorbance (412 nm) by nanodrop one instrument? 96 well plate ELISA reader can consider an option for that?

Juan Manuel Gallardo

Yes, you can use microplate reader. It is so fast and practical.

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