What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What is the reason of weight gain in TGA curve of a HDPE polymer sample ?

HDPE samples were aged at higher temperatures in the presence of water and CO2 than the TGA test was performed in Nitrogen? Weight gain is seen in the curve? What could be the possible reason for...

16 January 2021 5,644 2 View

What suitable boundary for stream function in inlets & outlets with COMSOL code?

As it has known the Dirichlet boundary condition is suitable for no-slip wall boundary for the stream function in COMSOL. I need to ask if I have an inlet and outlet I need to look for another...

02 January 2021 6,255 4 View

How to modify an article's "field of expertise" or "subspecialty"?

Greetings; I've recently published a preprint for a COVID treatment RCT, which appeared under "Dental" specialty instead of "Infectious diseases (ID)". This is an issue as it doesn't appear under...

14 December 2020 6,614 4 View

Hello, What kind of statistical Analysis test can be used for the survey questions with four different options?

I have conducted a survey which includes two groups (1-Normal people, 2-Deaf and Dumb), and the questionnaire had a category of questions with 4 different options and to answer only tick one. I...

01 December 2020 1,147 3 View

Who can i take a smear from inhibition zone?

I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...

29 November 2020 3,165 3 View

How can I prepare a stock solution from alcoholic extract of peganum harmala seed ? And how prepare multiple concentration ?

I used this method to make concentrations, by using distilled water to make concentration like 25mg/ml 25mg of extract in 1ml of distilled water and so on ....

29 November 2020 2,933 4 View

Survey on smart supply chain risk assessment?

Hi Folks I am working on my thesis for my Master's degree completion. I have produced a survey on the above topic. I need some expert's opinions and recommendations for the questions of the...

02 November 2020 4,870 3 View

What are the models of artificial intelligence based cars in automobile industry scientists are working currently with sustainable approach?

The question also includes the assurance of safety in the self-driving cars and the expected cost of selling. And what kind of quantum model will be more suitable in terms of physical realization?

29 October 2020 5,352 3 View

What are some concrete examples of time-fractional Navier-Stokes equations?

The introduction of time-fractional derivatives in the Navier-Stokes equations goes back to Lions and was recently extensively studied by many authors. When motivating such problems authors tend...

26 October 2020 9,642 4 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Why are my ARPE-19 cells exhibiting this morphology?

Hello! I am culturing ARPE-19 cells (passage 25) in DMEM + Sodium Pyruvate, Glutamine, 1% FBS and observing this phenotype in my cultures. These have been in culture for about 2 months and are...

12 May 2020 8,499 1 View

Alternative Media for Expi293s (Currently Using F17)?

Does anyone know of a more affordable alternative to F17 media for 293Expis? We currently supplement with L-glutamine and Pluronic F-68.

10 February 2020 637 1 View

Why L-asparaginase enzyme could give low cytotoxicity results in vitro on an anticancer experiment?

I'm currently working on my graduation project on the anticancer potential of E. coli L-asparaginase enzyme on mcf-7 along with other chemotherapy drugs. Previous references did prove cytotoxic...

10 November 2019 2,447 3 View

SDS-PAGE of a 410-kDa protein with pI 10.44?

Dear colleagues, I am working on a protein with peculiar characteristics. It is reasonably abundant in stem and cancer cells: - Size: 410 kDa - pI: 10.44 - Unusually rich in isoleucine and...

18 September 2019 321 2 View

SDS-PAGE of a 410-kDa protein with pI 10.44?

Dear colleagues, I am working on a protein with peculiar characteristics. It is reasonably abundant in stem and cancer cells: - Size: 410 kDa - pI: 10.44 - Unusually rich in isoleucine and...

08 September 2019 7,759 2 View

Any recommended handling method for neuroblastoma SH-SY5Y cells?

Good afternoon, I have some question related to the handling method for neuroblastoma cells SH-SY5Y. First, I grew the cells with EMEM medium (10% FBS and 1% Penicillin/Streptomycin). The cells...

07 August 2019 8,334 9 View

Any suggestions for PBMC/monocyte culture conditions (I get cells to attach well after isolation but then they die the next day)?

I have tried isolating PBMCs (looking to culture monocytes and differentiate into macrophages) from human blood using Ficoll gradient. I get a good amount of cells that attach. After 2-3 hours I...

06 July 2019 714 6 View