I am looking to find a series of commercially available antibodies for western blot analysis on human tissue covering the expanse of the tau protein (i.e. antibodies detecting the 6 isoforms- both native and phosphorylated forms).
I am doing extensive literature searches and the www.alzforum.org website has been helpful, but I wanted to post this to see what people are currently using in the lab and what they believe is the biggest bang for the buck.
I've heard from many that there are certain tau antibodies to stay away from when doing Western blots on human tissues (most literature out there is on rodents). Any aid would be greatly appreciated. So far in my repertoire I have AT8, T231, HT7, and PHF-1. Thank you in advance.
I had used p-Tau antibody (Ser 404) (Santacruz Biotechnology, Santacruz, CA). It works great for ICC
Thank you all for your helpful suggestions, after posting this one of my former colleagues pointed me to the following paper:
Title: Identification of amino-terminally cleaved tau fragments that distinguish progressive supranuclear palsy from corticobasal degeneration Author(s): Arai, T; Ikeda, K; Akiyama, H; et al.
Source: ANNALS OF NEUROLOGY Volume: 55 Issue: 1 Pages: 72-79 DOI: 10.1002/ana.10793 Published: JAN 2004
In Table 2 it lists a series of antibodies with a variety of binding sites- so I am using a smattering of these to cover the entire tau protein (not just specific phos. sites).
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