Hanieh,

Yes, for your gene you may simply add GCC ACC immediately before the ATG.

if You want a good expression of nanog you must add a Kozak séquence before ATG..

Hi Hanieh,

If you can not add the final G of the kozak sequence, just don't. You can simply go for GCC A/GCC then ATG and finally your gene. You will have translation in any case. You can check this paper if you want to know more about kozak sequences : http://nar.oxfordjournals.org/content/15/20/8125.long

Best