Hi Loris,

Here is the website of Dr. Evenson's company. You can directly order from them.

https://www.scsadiagnostics.com

Thank you for the advice Burak. I had already been on the website but it seems that we can only order SCSA Test as a patient. I'm in fact interested in the SCSA Software only, just to translate my cytograms of native vs fragmented DNA in a Total DNA Stainability vs DNA fragmentation index as required in the SCSA test.

Ask Prof. Heinrich Bollwein [email protected]

Kontakt: Direktor

Klinik für Reproduktionsmedizin

Departement für Nutztiere

Vetsuisse-Fakultät Universität Zürich

Winterthurerstrasse 260

CH-8057 Zürich

Www.scsadiagnostics.com directly

Hi

we used FCSExpress from DeNovo using the parameter math option to do the calculations.

We too

Dr Evensons technology is a licenced agreement and so requires payment for each use. I would stick with available interpretation sorftware unless you wish to offer SCSA or SDSA (TM) comercially. In this case you would then have to apply for the licence.

Thank you very much, all, for your answers, Indeed it helped me.

The software you need to analyse your FACS results is a list-mode software. Evenson's company is one source (if you can get them to answer your inquiry). Other options are Listview from Phoenix Flow Systems, FCS Express from De Novo, and Winlist from Verity.

Hi everybody,  I refresh a little bit the topic as I'm trying to figure how to analyse SCSA results with softwares different from the official SCSA-soft.

Indeed some of you gave me the advice to use FCS express, unfortunately this is not an open access soft and I only can download trial version. Thus I would like to understand exactly how the raw datas (I mean the cytograms red fluorescence  vs green fluorescence) are treated to obtain 1024 x1024 cytograms  or to calculate  the % DFI  as descibed by Evenson et al. My objective is to be able to treat these kind of data on Flowjo  for example?

Cordially

Hi Loris,

If I am not mistaken, you can obtain the 1024 x1024 cytograms by choosing lin mode instead of log mode (when you do the graphs). Then, you have to create for each sperm a new variable called DNI fragmentation index (DFI) by this formula: Red fluorescence/total fluorescence. I do remember using the FCS Express for this, and the math option (but I do not remember how exactly).

Have fun with your data!

Eva Mocé

Hi Eva,

Thank you for your answer. I will try to find if this function is available with our analysis software. Indeed I heard about the math option on FCS express but we don't have access to full version of this soft.

Thank you for the advice

actually, the parameter math is not so easy to do the calculations. I think you can use the free Flowing software, too.