I Traditionnaly work with germinal cells or fibroblasts but I have blood and bronchoalveolar lavage from mice that we would like to analyse on flow cytometry (monocyte population). However, the samples have been fixed one month ago and the morphology of the samples have been altered as FSC/SSC cytogram does not allow to descriminate Monocyte Population.

Do you Know a gating strategy for monocyte population that allow to analyse such altered samples. Do you think CD115 staining would be enough to gate the Monocyte population.

Cordially