Hi there,

I intend to remove carbonate from my %36 guanidinium carbonate solution meanwhile I am planning to get flow through guanidine base solution from SPE cartridge.

For this purpose I supplied Strata SAX SPE format resin from Phenomenex and it is consist of 500mg sorbent mass with 6 ml volume. I aware that I need to adjust meq capacity of the solution before loading to SPE due to high recovery retention of carbonate. At this stage I have several concern;

1- is the SAX proper resin for carbonate retention (removal)

2- I've learned that SAX from phenomenex has 0,9meq/g capacity. which loading volume (without changing the %36 w/v concentration) is the correct one and how should I calculate my loading solution meq/ml to confirm high recovery retention of carbonate anions...

3-SAX resin was supplied as counter ions chlorides were attached to Quarternary amine moities. At equabiliration steps should I change these counter ions with acetate or formate for getting more selective retention of carbonate anions ?

Thanks in advance...