I was analysing a sediment extract for PAHs using GCMS. The sample was extracted using PLE and cleaned up using SARA fractionation using three fractions: F1, Hexane (5 mL), F2, DCM (5 mL), F3, methanol (5 mL). GCMS shows that some traces of PAHs present in F1 and majority in F2. Some targets peaks were comparable in F2 and F3. However, deuterated PAHs were present almost equally in F2 and F3. PAHs present in F1 can be explained by being the ones that are relatively more nonpolar such as naphthalene. Presence of some analytes in F3 may call for higher volume of F2. But what could be the cause for the unusual behavior of the deuterated PAHs?