I am trying to stain organoids and where I am getting results with different staining protocols, I do noticed difference between protocols, especially when it comes to permeabilization component. Some seem to prefer Saponin and Others Triton-X100, or even both.
I am trying to find a nice paper or some more clear information about why I would consider using the different permeabilization components. Furthermore, I was wondering maybe that for organoids, that normally need longer permeabilization steps a component like Saponin might be more benificially seeing it does not effect protein structure.
Any information about this topic and papers I could read that could give me a more clear answer would be appreciated.
Check https://www.rndsystems.com/resources/protocols/flow-cytometry-protocol-staining-intracellular-molecules-using-detergents
https://applications.emro.who.int/imemrf/Avicenna_J_Med_Biotechnol/Avicenna_J_Med_Biotechnol_2014_6_1_38_46.pdf
Also check https://www.mdpi.com/2076-0817/10/4/431/htm
https://www.frontiersin.org/articles/10.3389/fcell.2020.618552/full
Check also on this paper
Article Tissue clearing technique: Recent progress and biomedical applications
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