Balaji B.

5 Questions 5 Answers 0 Followers

Questions related from Balaji B.

Is the guide RNA (gRNA) designed for gene knockout has possibility of binding to messenger RNA (mRNA) of the target gene?

In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...

26 December 2023 593 1 View

Sanger sequencing giving multiple peaks after few base pairs. What are the possible reasons? How can I confirm that? Suggest me way to get the seq ?

I am analyzing the gene sequence of my target gene in Brassica juncea. In that there is some ambiguities in the gene sequence which is appearing after few clear peaks in the sequence. I am...

28 June 2023 9,908 1 View

Homology modelling Vs. Protein threading?

On the above mentioned modelling methods, which one is suitable for protein structure prediction for protein/gene families? Suggest some highly reliable modelling methods and software used for it.

13 June 2023 1,447 7 View

Suggest some good laptop models suitable for working in Bioinformatics ?

I need a suggestion about laptop hardware configurations or models available in India market which are recommended and versatile to carry out in-silico experiments and dry lab works.

05 July 2022 4,236 0 View

How Warburg effect helps cancer cells?

Even in the presence of large amount of oxygen and functionable mitochondria, cancer cells follow anaerobic respiration to generate the ATPs to meet it's energy demand. It sounds like they are...

01 January 1970 3,018 3 View