Sample specific house keeping genes for qPCR

Kamesh Ravichandran @Kamesh-Ravichandran

01 January 1970 0 4K Report

I am looking for an house keeping gene for nuclear extracts and cytoplasmic extracts from mouse and human cells. The purpose is to check whether we have done our nuclear and cytoplasmic extractions without cross contamination. Any suggestions!

Badges
Science topic

More Kamesh Ravichandran's questions See All

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

What does an "Error 111" mean on an AKTA Explorer?

Dear Colleagues, I would be grateful if any of you can advise me on what an "error 111" is on an AKTA Explorer. The manual suggests that the instrument be turned off and turned on. That does not...

13 June 2024 2,961 1 View

How to calculate unit of enzyme, enzyme activity and specific activity from absorbance?

Cellulose is broken down by the enzyme cellulase, to find the cellulase activity and unit production by the microorganisms, the microorganisms were cultivated by using cellulose substrate CMC and...

13 March 2024 1,390 0 View

Choosing Good Research Topic Will Help to get Good Job?

For Msc Data Science Final Project

13 January 2024 5,183 1 View

Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...

10 December 2023 5,680 2 View

Which is a better one for predicting outcomes like tax cases - binomial regression or Logit regression?

Hi, I have some confusion as to which one is better for outcomes as a model using binomial regression or logistic regression. currently i am working on judicial decisions, outcomes in tax courts...

01 December 2023 4,292 3 View

How to design a reverse primer for poly A tailed microRNA?

Hello, I am working on microRNA expression studies on Regeneration. I isolated RNA from my sample and converted it into cDNA using Poly A polymerase and Reverse transcriptase enzyme. Previously I...

18 August 2023 3,898 0 View

What are base papers to research on elliptic curve cryptography. Any suggestion please?

I need papers for that

19 June 2023 9,098 5 View

How to calculate the degree of crystallinity for Polypropylene with multiple melting peaks?

I've been trying to study the degree of crystallinity of Polypropylene using DSC. In the heating curve I find two melting peaks - one at around 150 and the other at 160 degree Celsius. I think...

22 November 2022 5,882 0 View

What dilutions of serum sample should I use in ELISA?

I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...

18 February 2022 10,013 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View