RRNA-seq repeat sequence analysis?

25 April 2018 1 3K Report

Can anyone suggest bioinformatic tools to analyse expression levels of rRNA as the alignment software just throws the repeat sequences in ambigous reads

Ammar Khursheed

SINA tool https://www.arb-silva.de/aligner/

Badges
Science topic

More Ataul Haleem's questions See All

How to avoid formation of coagulation in core-shell emulsion polymers?

good morning, I'm synthesizing emulsion polymers via core-shell technology, but I face problems during the synthesis as I find large amounts of coagulation in the form of sand-like particles. Why...

20 May 2024 9,905 5 View

Do you believe that the main source of climate change is greenhouse gases?

the scientific consensus is that greenhouse gas emissions from human activities are the primary driver of the observed changes in global climate patterns.

03 December 2023 5,154 16 View

How can i calculate the C=C in the oils ?

I'm running Epoxidation of Soybean oil to get EBSO plasticizer, but I don't know how to calculate C=C molar ratio of the oil to further use it calculation of Molar ratios of formic acid to peroxide..

06 October 2023 5,530 0 View

What is the recommended temperature for transport the phage laysat and can i transport at room temperature?

temperature, storage, bacteriophage

12 July 2023 7,779 1 View

How can i synthesize the following compound POLY(ETHYLENE-CO-GUANIDINE), HYDROCHLORIDE?

this is Water Decoloring Agent has high color removal of dye wastewater.

08 July 2023 8,410 0 View

What is the approximate sputter deposition rate of carbon for a conductive coating?

We want to have a thin (~10 nm) conductive coating of carbon (graphite) on Si samples. While a C sputtering target is available in market, manufacturers don't spcify its (i.e., C) deposition rate...

21 June 2023 9,466 3 View

How can I make storage the phage lysate from spot assay?

storage phage lysate and spot assay test

24 May 2023 3,286 0 View

What is the soft agar which is used in double layer agar to determine the titer of viruses?

soft agar and double layer agar method

08 May 2023 8,196 2 View

How to detect the epoxy value of epoxy soybean oil?

I made epoxy Soybean oil but i want to know how to detect the epoxy value of the product.

24 February 2023 4,464 0 View

How to determine the methyl ester content in the biodiesel?

I'm producing biodiesel and I want to Know how to determine the ester content by using titration method not instrumental method because instrumental method is not available.

03 January 2023 1,110 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View