I am using Qiagen RNeasy Midi kits to extracts RNA from various rat brain ROIs. I have a lot of samples to process, and would ideally like to do the tissue homogenisation step separately from the RNA extraction. e.g. freeze down the homogenate to be thawed and used another day.
Is this safe to do? Any idea on how badly it will affect RNA yield if at all?
And, if so, what is the best point to do it? Should I freeze the homogenate in RLT buffer+B-ME alone, or should I add the ETOH first?
Any other suggestions or tips are very welcome!
Thank you :)
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