RNase treatment for woody species

11 November 2012 1 4K Report

any protocol for eliminating RNA after DNA extraction without using phenol?

ya you can try with CTAB method with >1% PVP and .3% B- marcaptoethanol in the extraction buffer. Here you can use RNase instead of using B- marcaptoethanol and if you want to avoid phenol step, use Proteinase K. Best f luck.

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