How to perform quantification of pericyte area in ImageJ?

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How to conduct the NMR study of a nano-composite film?

Solid state NMR study

03 July 2024 422 1 View

What will happen if hypoxia chamber gets turned off between P7-P12?

Dear all, I want to check the effect of hypoxia on retinal angiogenesis in mice pups at P18. For the same I placed one cage in hyperoxia chamber (75% oxygen) with mice pups from P7-P12. I planned...

18 August 2023 6,554 0 View

Is it possible in any genetics condition, only mother is carrier for mutation and child has Homozygous mutation for same variant ?

Father- Normal Mother- Carrier Child - Homozygous variant

18 April 2023 2,349 2 View

What would be the research gaps under Tourism and AI?

Hi, I am an undergraduate student and I'm trying to write a thesis about tourism and AI but I can't seem to figure out the research gap... if anyone has any suggestions they are more than welcome...

07 December 2022 4,672 3 View

FsQCA test calibration and truth table query..?

Dear all, I have to apply FsQCA test on my data, I hv few queries in fuzzy set data.. 1. Whether we can score during calibration of data matrix to any condition as 1 or it should be less than...

02 September 2022 4,307 0 View

How do I quantify the cell area and cytoplasmic v/s plasma membrane distribution of tight junction proteins in bEnd.3 cells using confocal microscopy?

Hi, I want to quantify the cell area of bEnd.3 cells (mouse brain endothelial cells) using confocal microscopy by ImageJ. Most of the available protocols need to ignore neighbouring cells where...

13 October 2021 6,486 8 View

Could u help with different techniques installed across the world to prevent jelly fish ingress problems in sea water intake syts desalination plant?

jelly fish ingress is a major problem affecting the sea water intake systems leading to plant outages in power plants and desalination plants. can anyone through some insight on installed and...

15 December 2020 6,862 4 View

What is a Herzog plate and what are its uses?

They are used in optical systems before and after the electrostatic deflector plates. But it is not clear what is the purpose of using them and why???

28 October 2018 2,248 0 View

Whether to Run Bivariate Johansen co-inegration test is valid?

Dear all, In literature we see Johansen co-integration test is multivariate test. We find papers on Bivariate Johansen co-integration also. some experts say It is a multivariate test so should be...

09 October 2018 384 0 View

Is it necessary to consider mean equation coefficient's significance while testing for any GARCH, EGARCH or BEKK GARCH model?

Dear all, I want to know that when we run any GARCH model we firstly estimate a mean model and do its residual diagnostics to see arch effect. if arch effect found then run GARCH models. mean...

04 October 2018 3,382 10 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Gowtham Kumar Annarapu

Hi Teena,

How are you?

I think you better off to use Nikon (if you used Nikon imaging system)/Imaris softwares where you have all these measurement options or can use photoshop to measure the same (https://www.youtube.com/watch?v=vdnd88baxV8&t=673s).

Teena Bhakuni

Hi Gowtham Kumar Annarapu . I am good. How are you? I have used Nikon iamgining system. Let me go through the softwares you have mentioned. Thanks again !

Best

Teena