Teena Bhakuni

5 Questions 6 Answers 0 Followers

Questions related from Teena Bhakuni

RNAscope of whole mount mouse retina?

Hi All, I have been trying to perform RNAscope in whole mount retina of mice at postnatal day 6. Since there are a lot of incubation and washing steps, I need to transfer retina between 2 mL...

20 February 2025 6,135 0 View

What will happen if hypoxia chamber gets turned off between P7-P12?

Dear all, I want to check the effect of hypoxia on retinal angiogenesis in mice pups at P18. For the same I placed one cage in hyperoxia chamber (75% oxygen) with mice pups from P7-P12. I planned...

19 August 2023 6,577 0 View

How to perform quantification of pericyte area in ImageJ?

Hi All, I need to quantify the pericyte area and pericyte coverage in mouse retina samples. Can anyone help me with the quantification steps on ImageJ? I plan to use NG-2 staining for pericytes...

11 November 2022 8,840 2 View

How do I quantify the cell area and cytoplasmic v/s plasma membrane distribution of tight junction proteins in bEnd.3 cells using confocal microscopy?

Hi, I want to quantify the cell area of bEnd.3 cells (mouse brain endothelial cells) using confocal microscopy by ImageJ. Most of the available protocols need to ignore neighbouring cells where...

14 October 2021 6,508 8 View

Is collagen coating (or any other ECM) required for bEnd.3 cells immunofluorescence staining?

Hi...I want to do immunofluorescence staining for junctional proteins in bEnd.3 cells. Can anyone suggest me if I need to coat the cell culture plasticware with collagen? I am seeding cells on...

08 July 2020 7,489 0 View