Hello,

Our lab is attempting to do RNA-sequencing of primate brain tissue. I am wondering if anyone knows of a published protocol that highlights the dissection methods used and how to determine the preferred ratio of tissue to trizol before homogenization.

Additionally I am wondering if glass beads are preferable to the steel beads with larger tissue samples as the steel beads are quite large and do not seem to be homogenizing well.

Thanks for your time,

Mélise