In RNA isolation (from frozen blood samples) using TRI reagent , can i use chloroform that is stabilized with ethanol?
You should use chloroform for molecular biology from Sigma which is using amylenes as stabilizer (sigma C2432), it works well and is suitable for extraction of nucleic acids.
Suheir,
Trizol should be sufficient to keep your samples stable and clear for the short term.
Could you provide some information on why you want to use the chloroform/ethanol?
i want to use it for RNA isolation from frozen human EDTA blood samples (samples were frozen without addition of any RNA stabilizer). The protocol of isolation requires a chloroform step to separate the sample into phases and then requires a step of isopropanol and finally washing RNA pellet with ethanol. The chloroform i found in our lab is from SIGMA and contains ethanol as stabilizer , thus i wonder if it would be suitable for this protocol.
Alcohol will interfere with your RNA isolation process. So it is not advisable to use chloroform containing ethanol as stabilizer. But it also depend on how much conc of ethanol present in the chloroform....
So if you able to do trial and error with regular sample and then go for target samples...
You should use chloroform for molecular biology from Sigma which is using amylenes as stabilizer (sigma C2432), it works well and is suitable for extraction of nucleic acids.
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