Hi Jennifer Maurer

Freeze-drying the tissue sample is a good option for downstream analysis of RNA. How did you freeze dry your tissue, did you use a cryoprotectant like trehalose or sucrose? If you have used a cryoprotectant then you can proceed straight away with your RNA extraction by homogenizing your freeze-dried sample in TRIZOL and use chloroform and isopropyl alcohol to precipitae your RNA. Check the below-mentioned article, the principle is same for tissues.

Article Lyophilized human cells stored at room temperature preserve ...

Hi there

I routinely used human heart biopsies preserved at -80ºC for RNA isolation. These RNAs can easily be used for NGS. We freeze the tissues immediately after collection in the surgery and preserved in an RNA extraction buffer with beta-mercaptoethanol.

In heart tissues preserved by this way you can easily reach RINs of around 7-8. Sequencing results are very good.

Hope it helps.

Best

Hello,

Yes you can if the sample is well preserved, I have described a manual extraction protocol using magnetic beads "Dyna Beads" with a very good performance even with less preserved molecules

Here is the link of the article.

Article Direct Diagnosis of Echovirus 12 Meningitis Using Metagenomi...

Good luck for your manipulations

hi. is the freeze drying same as high vacuum drying?

also any advice on using either one of both ?

thanks

Yes, Zaidah, both are the same thing, samples have to be frozen all the time during drying and vaccume process