How can we estimate sample size for a moderated mediation analysis with more than two mediators?

How can we estimate sample size for a moderated mediation analysis with more than two mediators? I am looking for scripts, macros, syntaxes, that allow us to estimate sample sizes for research...

15 February 2021 6,813 3 View

In your opinion, which is the best software for reviewing Papers? Why?

How effective and helpful is it, really.

25 January 2021 6,988 73 View

Have you cloned a PCR product containing 2X2A sequences into a vector that contains another 2A sequence?

Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...

17 December 2020 5,218 3 View

Can you suggest a mini research topic?

What are the possible topic in this time except pandemic? Thank you in advance for the suggestion.

06 December 2020 5,380 7 View

Contributions to Nanomaterials Special Issue "Porous Metal-Organic Framework Nanoparticles"?

20 October 2020 248 2 View

CRISPR-Cas9: Are there any alternatives to the use of fluorescent PCR and capillary electrophoresis?

We are trying to find alternatives to the use of fluorescent tagged primers and capillary electrophoresis to access somatic activity of the target gene. Thank you!

25 September 2020 4,761 3 View

Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability?

I have a question for KLH experts. Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability? Is a T- cell expressing KHL- or...

24 September 2020 2,931 1 View

To what extent does technology influence energy efficiency in relation to sustainable development?

Hello, I ma an undergraduate student studying environmental science and about to start on my dissertation and I am still thinking around a topic.I am interested in technology and sustainability...

24 August 2020 3,930 11 View

Can someone please help with inputting PLINK assoc.logistic output files into METAL to perform a meta-analysis?

I need to perform a meta-analysis of 2 GWAS studies and I need assistance with the input study files (plink --assoc.logistic) as well as the output meta-analysis file (.TBL). - Firstly, I am not...

07 June 2020 5,831 1 View

Can we make a Systematic Review when we get zero papers to answer our research question?

I don't seem to find anything published (or unpublished) to answer my research question. Broadly, when I search for "PTSD + Virtual Reality + First Responders" (and all the variants these words...

11 March 2020 3,349 7 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

How to measure abundance in an LC-MS/MS experiment?

Is the chromatographic peak a valid measure of a compounds` (relative) abundance in LC-MS data? We have recorded three MS/MS spectra; their retention time falls into a specific chromatographic...

13 January 2021 6,685 6 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

Metabolomics Data Normalisation?

Hello, Setting: We have a rather large dataset consisting of somewhat 500 samples containing metabolomics data. The data was obtained by analysing dry capillary blood using HPLC methods, yielding...

04 January 2021 8,773 5 View

Analysis of significance by using R programming ?

Dear All, I have the data of metabolic pathways in case and control, well there are pathways which are represented several times due to the involvement of reactions in case, well now I would...

09 December 2020 4,494 5 View

How to identify possible metabolite from chromatogram?

We have done the analysis for a pre-clinical study and we have observed additional peaks in the chromatograms. We think that these peaks are metabolites. The concentration of the additional peaks...

02 December 2020 6,134 10 View

How to analyze two different time point metabolomics data?

Hello, here I have a question of how to analyze two different time point metabolomics data. eg, dataset 1 was done in June, and dataset 2 was done in October. What kind of methods should I used...

30 November 2020 5,495 2 View

Would anyone be able to recommend some online courses/ r videos for metabolomic analysis?

Hi everyone, I would like to improve my computational skills in r, particularly in the field of metabolomics (both targeted and untargeted analysis). I think using R will make my life much...

19 November 2020 1,171 7 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

Is it possible to use lyophilized blood plasma for "omics" analysis?

Has anyone used or read about blood plasma lyophilization (my interest would be for safe transport/conservation of samples over long distances) for posterior analysis of metabolomics/...

26 October 2020 7,296 7 View