RET immunohistochemistry in NSCLC?

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Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to correctly specify a multi-group latent change score model in Mplus?

Hi all, I face problems in specifying the correct Mplus input for running a multi-group comparison of a latent change score (LCS) model, including a PRE and POST measure with 3 indicators for...

21 May 2024 4,578 1 View

Are there any research data on the dangers and risks of inhalation of titanium dioxide E171?

It is in detail the repeated daily exposure over years to a powder containing about 50% of titanium dioxide E171, sprayed with compressed air in the breathing zone. What reactions from the lungs...

13 November 2023 5,530 3 View

Does anyone know this kind of cell culture contamination?

First, there are bubbles with small black dots inside visible. The next day those bubbles disappear and the rod-like structures are present in our cell culture, which are fast growing but not...

26 June 2023 4,139 1 View

Do M-CSF bone marrow-derived macrophages express F4/80?

F4/80 is used as a marker to identify murine tissue/peritoneal macrophages. In our working groop we isolate monocytes from rat tibia and femur and differentiate them subsequently into bone...

13 March 2023 8,339 3 View

How to get an estimation of independent variables in SEM?

Hi SEM and Mplus community! I think this question is rather basic but unfortunately I can not find a solution to this issue: In Mplus 8 I estimated a linear second order growth curve model with...

05 March 2023 2,698 3 View

How do I choose the correct test of significance for experimental data?

I have 5 biological samples, each tested at two dilutions (4 technical replicates each). I am using a standard curve to convert the fluorescence units into micrograms of protein and normalizing by...

25 April 2022 2,474 7 View

Are there any good bi-directional DC power supply as battery simulator?

Hey, I'm looking for some precise (µV/µA) and fast (

10 October 2021 2,787 3 View

What could be the reason for a poor primary renal fibroblast cell growth ?

Hello all, I'm trying to isolate and expand human primary renal fibroblasts from nephrectomy samples using an explantation technique. This technique has already been used successfully in my lab...

19 September 2021 4,766 5 View

Flow cytometry marker for human keratinocytes?

I would like to use flow cytometry to characterize the cells of a Single Cell solution of human skin samples. Does anyone have a tip which marker is suitable for keratinocytes? Thanks!!

12 September 2021 5,112 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Ethylene glycol is newtonian or non newtonian fluid?

Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.

09 August 2024 2,111 2 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View