Why do some full text requests not allow me to respond to the requesting person?

Over the last year, when I have clicked on 'View the Request' in the notifying e-mail for a full text request, I have been taken to the paper in question but there is no request visible and thus I...

16 January 2021 7,558 1 View

How can I accelareate dehydration of mouse lung with 10% sucrose for frozen section?

09 August 2020 4,967 1 View

How to visualize output files of Quantum Espresso?

I am a starter of Quantum Espresso. I want to visualize the output files from Quantum Espresso. Primarily, I want to see atom configurations and trajectories from .xyz files and .path files....

20 June 2020 8,755 1 View

Can anyone help me about errors of Process Macro multiple mediator analysis with model 4?

When I start PROCESS I get two error messages: ->Error 12410 Source operand for EVAL is not balanced. > >Undefined error #12354 - not found in file .. How can I solve this...

26 March 2020 6,738 2 View

How many mice are sacrificed to obtain 1 mg antibody with the ascites method?

To get an idea about the animal welfare impact of my planned experiments and to perhaps make adjustments in the antibody selection, I was wondering: How many mice are sacrificed to obtain 1 mg...

08 March 2020 2,350 3 View

Which MCDA-method (and/or software) is most suitable for a decision with alot of scenarios?

For my thesis, which thematizes a GIS-based industrial site selction in Europe, i am in need of a method to integrate all of the european NUTS 2-regions (253 units). The methods which i had in...

29 February 2020 6,743 5 View

Why doesn't my iron crystal format an isosceles triangular twining boundary?

I have simulated iron crystal (BCC) in Lammps using many different potential files in order to make an isosceles TB. I modeled the TB by rigorously calculated the offset distances of the two...

02 September 2019 9,403 1 View

LCMS microcolumns: POROS 50 Reversed R1 vs R2?

I am looking for material to make my own microcolumns/ in-tageTips for peptide purification before LCMS-analysis. Earlier i have used C18-disc and POROS 50 R2 reversed powder (column material)....

31 July 2019 1,453 4 View

'First Principle Reasoning' / 'Physics Thinking' for radical innovation - will it emerge as a viable research area?

Is anybody interested in or already doing research within the field of 'First Principle Reasoning' / 'Physics Thinking' for radical innovation? It's a mental model entrepreneur Elon Musk is...

02 April 2018 7,616 4 View

What are success factors to implement eco-innovations?

08 March 2014 1,110 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View