Resuspend salivary proteins in 1X Coupling Buffer???

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How can I generate a population with a fixed correlation coefficient in R?

I want to generate an artificial population using two variables (say x & y) with a fixed correlation between x & y. Which package/command in R software can help in this?

18 January 2021 2,545 9 View

How to improve the organisation of my manuscript from poor to good?

Several time i received this comment from the reviewer that your manuscript organisation is poor. Please suggest me how to improve it.

24 November 2020 1,719 3 View

What can i do to extract antimicrobial compound from bacterial medium supernatant if do not know the nature of antimicrobial compound?

I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...

21 September 2020 6,869 3 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

How can we perform a GWAS analysis of a qualitative trait through GAPIT?

Hi,I am doing GWAS analysis on both quantitative and qualitative trait through GAPIT 3.0. Code run well for Quantitative traits but shows following error in qualitative trait Error in...

18 July 2020 9,541 5 View

Can anyone suggest the best tool to perform Optimization (Such as PSO, GA, ACO) in vehicular ad-hoc network?

I am looking forward to optimizing the data delivery in vehicular networks. Can we use python or MATLAB OR NS-2/3.

29 June 2020 6,380 10 View

Deep Learning Model?

model.add(Dense(20,input_dim=13,activation='relu')) model.add(Dense(10,activation='relu')) when we write code like this to add hidden layers in neural network then : 1. what this 20,10 values...

27 June 2020 1,244 4 View

For Scanning electron microscopy based trichome analysis of the leaf samples fresh samples can be preserved?

For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...

01 June 2020 5,665 1 View

Can anyone refer to any topic (or any research article) related to "Machine learning with the vehicular ad-hoc network"?

21 April 2020 1,272 3 View

Synthesis of cis-3-bromocyclobutanecarboxylic acid ?

Dear All, Any reference and paper for synthesis of cis-3-bromocyclobutanecarboxylic acid ? If any suggestion for preparation ? So please share your thoughts.

09 March 2020 9,618 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

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How optimize the Size Exclusion Chromatography?

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01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

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01 March 2021 9,015 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

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28 February 2021 5,415 1 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

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26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Alemu Tekewe Mogus

You need to consider the effect of the pH and/or salt concentration of your resuspending buffers. What is the buffering agent in your coupling buffer? Sodium acetate or ammonium bicarbonate buffer?

Kuldeep Giri

Thank you Dr.Alemu Tekewe for replying. Buffering agent in coupling buffer are 100 mM sodium acetate, 150 mM sodium chloride, pH 5.5.