Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

Can anyone suggest how to remove H&E stain from the stained slide?

Dear friends i have only H&E stained slide and i have to do other special staining from this slide only. kindly suggest me the procedure for complete destaining of this slide.

13 December 2020 634 4 View

While doing gradient PCR for my gene of interest, Iam getting multiple bands in including a amplified band lesser than the size of my interested gene?

I have checked my primer sequences and they are particular with my gene. What could be the reasons for this?

25 September 2020 3,398 0 View

How many cells would be good to perform Chip seq for FoxP3 binding domain? Which fixative would be good for the same?

We are trying to find FoxP3 interactive domain in WT vs KO mouse regulatory T cells (Treg) through Chip Seq analysis. I am unable to get anything after pulldown although sonitation was good....

24 September 2020 244 4 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

Which professional society bodies in India related to Civil Engineering provide worthy periodicals for researchers/Engineers?

List out the best professional bodies which provide worthly and subject-related periodicals. Also, list out as per specializations like Structural Engineers, Geo-Technical Engineers, Environmental...

09 September 2020 1,151 11 View

How to go about designing primers?

I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...

24 August 2020 6,379 3 View

Does anyone aware of a easy way to extract data on the number of publications per year on a particular research area?

Is there any easiest way? I need data of my research area for my upcoming review article.

12 August 2020 3,826 4 View

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,713 2 View

Do you agree with the just issued Great Barrington Declaration regarding the response to COVID-19?

The short and to-the-point text is here and I for one gladly co-signed this declaration, just as I also co-signed the balanced approach statement issued in July 2020 by 23 public health experts...

04 October 2020 8,795 11 View

The meaning of protein phosphorylation to bioscience?

Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did...

23 July 2020 7,135 3 View

Is there any cause of pellet from the culturing media?

11 June 2020 3,585 3 View

How do you reduce the static forces in canon ball packing of fruits for transportation?

Examples of how to increase the contact surface area of the bottom area? Thank you.

25 March 2020 9,187 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

03 January 2020 3,565 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

31 December 2019 3,792 4 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

22 September 2019 2,889 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

08 September 2019 5,009 3 View

So what you think that either you will choose to develop a desktop application, mobile application or the application that can run on both platforms ?

As internet usage increase web application development become a challenge, because it is mostly customer requirement that web application must be usable on both desktop computer and...

07 August 2019 1,193 3 View