I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

How to measure porosity/diameter of electrospun nanofibers using imagej?

Hi all, I have SEM images of electrospun nanofibers and I'd like to measure its porosity/diameter. However I'm not sure how to do it with ImageJ or any other software. Can anybody help me please?...

27 July 2020 5,925 7 View

How important is it to check the psychometric properties of translated version of a gold standard scale?

There is a scale in English that has well established psychometric properties. I want to use the translated version of the scale in my study. Do I have to test all the psychometric properties of...

11 June 2020 3,951 14 View

Should solar irradiance be considered in Wh or kWh when designing a PV system in kW?

Hi, I am designing a PV system in kW and having a small confusion that should I be considering the solar irradiance in Wh or kWh in the PV calculations? Thank you.

02 July 2019 4,127 3 View

Can anyone provide a reliable protocol with tips and tricks for miRNA isolation from human plasma using Trizol?

Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...

13 June 2019 2,228 9 View

Can anyone help me understanding why am I getting jagged amplification curve for my miRNAs in plasma sample?

I have used mircury exosome serum/plasma kit along with trizol for extraction of my miRNAs from human plasma. RNA concentration was 623.8ng on nanodrop with 260/280 ratio of 2.12. For RT-PCR and...

11 June 2019 3,679 4 View

What is the best way to purify the ear clip genomic DNA from TaqMan DNA extraction reagent?

13 October 2018 3,427 3 View

Significance of pAkt/Akt and pErk/Erk ratio?

Hi, I'm testing the effect of a drug on cardiomyocytes. The treated cells are then used for Akt and Erk ELISA. Can anyone tell me how do I interpret the pAkt/Akt or pErk/Erk ratio? For...

10 September 2018 3,959 3 View

Insulin concentration in culture medium?

Hi all, to mimic the hyperinsulinemia, what concentration of insulin should be used in the culture medium of growing cell lines (for example L6/MIN6) ? Also what should be the normal concentration...

21 March 2018 2,255 2 View

How to find quantitative determination of fe2+,fe3+ ratio from xps?

In my sample fe deped BaTiO3, both Fe2+ ferrous and fe3+ Ferric ion i.e.,multi valence states present which identified from XPS. How to find fe2+:Fe3+ ration present in my sample? which method I...

20 May 2017 8,210 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

What is difference between In-line transmission (%) and Total transmission (%) value of optically transparent Ceramics?

I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...

20 February 2021 9,405 6 View