I do not have nanoscience background i read several research paper about biological synthesis of NPs but it was not mention there. Your suggestions will be highly valuable for me.
You must pay attention to two important issues, the first is to obtain the required nano material, and this depends on the method of preparation, which may require annealing. Another thing to pay attention to is the issue of the resulting particle size, in which annealing is a impotent factor.
When you synthezise nanoparticles of a material, you expect for precise qualities of that material (those nanoparticles). You measure the optical transmittance, the electrical resistivity, the crystalline structure (using XRD) , etc. If the crystalinity of the nanoparticles that you have obtained is not good then annealiing is necessary. Annealing improves the crytalinity and thus the other characteristics of that material is improved.
Dependendng on the synthesis process, dometmes, we need to proceed to the annealing treatments.
The case of chemical functionnalization with nanoparticles moderate annealing leads to eliminate residual solvent and impurities and more dispersed state of nanoparticle on the organic matrix.
that's a very interesting and important technical question. You can find a large body of relevant literature about the biological ("green") synthesis of a variety of nanoparticles right here on RG. Numerous interesting research papers about this topic have been posted by the authors on RG, many of them even as public full texts. Thus I strongly suggest that you search the "Publications" sections of RG for relevant references. Just search for terms like "green synthesis of nanoparticles" or "annealing of nanoparticles" and the click on "Publications". You will be surprised to see how many helpful papers are directly accessible through RG.
In general, annealing is not a necessary step after nanoparticle synthesis, but it is often employed to achieve well-defined particle size, crystallinity or physical properties. What is most often a necessary step is the so-called "calcination" which is required to convert synthesis intermediates into the final nanoparticles. For example, biological or sol-gel syntheses of ZnO nanoparticles often initially yield hydrated products such as Zn(OH)2 which are then transformed into ZnO by heating (calcination).
Good luck with your work and best wishes, Frank Edelmann
In nanomaterial synthesis, annealing is a necessary step as it result in alteration of the morphology i.e. shape and size of the nanomaterial and hence improves the crystallinity of the as synthesized nanomaterial by modifying the physical and chemical properties so that it can be used for technological purposes..