Is it ok if we give cells two heat shocks during transformation?

Shalini Shalini Chilka @Shalini-Shalini-Chilka

15 October 2022 0 3K Report

While doing transformation I kept cells(dh5alpha) at 42 for minute but according to the protocol it needs to be kept at 42 for 90 seconds so I kept my cells at 42 again after keeping them in ice will it affect transformation efficiency?

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