Isolated RNA from human blood sample using TRIZOL method got ratio 1.37 and concentration 369.3ng/ul . Is there any method to increase the ratio of the RNA.
Abida Bhat Extracted RNA with 260/280 ratio of 2.0 is generally accepted as pure. Abnormal low ratio in your case indicate that your extracted RNA is contaminated by residual ethanol, guanidine, or other reagent used in the extraction protocol. Try to dry your RNA palette well before dissolving in nuclease free water.
When the mixture is separated into a lower phenol-chloroform phase, an interphase, and a colorless upper aqueous phase, you are asked by the protocol to recover only the upper aqueous phase (RNA).
This step of the protocol is closely related to the final 260/280 ratio just as with the final RNA yield. If your sample is expected to have few cells (and consequently low RNA yields), recovering most of the aqueous phase is very important.
However, recovering most of the aqueous phase could ultimately involve recovering some of the interphase by accident and this is where the proteins in your sample might lower your 260/280 ratio.
Absorbance at wavelengths of 280 nm indicate the presence of proteins in your sample. The more proteins, the higher the absorbance, thus lowering your 260 (RNA)/280 ratio.
If RNA yield is not very important, my suggestion will be to work as carefully as possible during this step using 200ul pipette tips and recovering a "safe" amount (half) of aqueous phase that will ensure that almost none of the interphase was recovered with the aqueous phase.
Hope this helps.
-M
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