When the distance travelled by methylene blue dye dot from the umbo to the annulus needs to be measured in micrometers with an zero degree endoscope, how can it be done ??
In the age of Mutiresistant drug bacterium(s) and eukaryotic pathogenic organisms or superbugs, we are in a constant lookout for new treatments and especially antibiotic drugs. Two approaches, in...
23 August 2020 3,915 3 View
ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...
08 February 2020 9,015 6 View
I need to get rid of the ethylene vinyl acetate paste stuck onto a glass surface. What would be the best solvent to get rid of it?
09 August 2019 9,016 6 View
Certain individual cases are very interesting and the experience of managing could be shared through images with brief detail.
18 September 2018 7,651 3 View
Cancer radiation therapy.
11 September 2018 7,623 5 View
I have a assembly with around 300 parts. If a few parts are similar but their orientation differs is there a procedure to automate the meshing of all these parts.
22 April 2018 6,378 1 View
Children are the most vulnerable and voiceless in our society. How can we address the issue of child abuse and let their voices be heard? Should a child leave home being a victim of extreme violence?
07 April 2018 2,014 4 View
Do both are affected by initial glucose concentration in media ? I have taken initial glucose (substrate) in following concentrations (g/l) : 20, 40, 60, 80, 100. Rest other components of media...
05 April 2018 9,279 4 View
I run standard mixture on TLC plate. All standards showed with double band.
26 February 2018 3,292 3 View
I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?
10 February 2018 8,861 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
I am working on modeling of Direct Contact Membrane Distillation system with COMSOL Multiphysics software. I have some problems with the mass transfer part of the simulation because I don't know...
14 February 2021 379 2 View
This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....
08 February 2021 8,321 3 View
Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...
07 February 2021 5,591 4 View
Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...
01 February 2021 1,351 3 View
I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...
25 January 2021 5,736 3 View
I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...
24 January 2021 7,226 3 View