When the distance travelled by methylene blue dye dot from the umbo to the annulus needs to be measured in micrometers with an zero degree endoscope, how can it be done ??
I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...
24 July 2024 9,688 0 View
Hello there, i want to know what varibles should i use for the reinitialization parameter , as i want to track the solid deposition boundary with depletion of precursor vapors. how can i do it on...
03 April 2024 7,907 0 View
I am beginner in the nmr spectroscopy can anyone help me how to interpret 2d analysis and structure elucidation. analysis type cosy HMQC HMBC NOESY NOE ROSY ETC
21 November 2022 4,329 2 View
How to know day by day number of sample run by analyst in topspin nmr software
06 October 2022 378 1 View
Any one please provide the lead properties for steinberg - guinan constitutive model implemented in Ls - dyna as *MAT_STEINBERG.
03 October 2022 2,818 0 View
I am simulating a FMJ bullet impact on a helmet, which uses MAT162 model for simulating its damage. when i tried for a coarse mesh(nearly 5mm element size) the bullet does not penetrated the...
03 October 2022 2,686 1 View
How can I separate two DNA fragments of 3000bp and 3700bp using polyacrylamide gel electrophoresis?
06 September 2022 9,723 4 View
I am using vegetable oil as a carbon source for the bacteria and supplying air in the fermenter. During the course of fermentation, it is causing heavy foaming in reactor which is not getting...
06 July 2022 8,186 3 View
I am working on protein hydrolysates of fish and performing plastein reaction using alcalase at 60 degrees for 6 hours. Can anyone suggest me the concentration of enzyme in percentage or about the...
26 May 2022 1,862 0 View
A patient had bp of ,170 by 13O and could hear his heart pounding. Pulse study Wood pulse was strong,and moving back vwards Understanding and findings Strong pulse indicates.excess Chi Going...
29 March 2022 4,000 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
Regeneration Studies for the PVDF membrane and PVDF/CuO nanocomposite membrane were carried out by chemical cleaning.
07 July 2024 7,033 0 View
Using confocal imaging, I'm investigating the effect of a particular compound on mitochondrial membrane potential in live cells. I've stained the cells with varying concentrations of the compound...
29 June 2024 7,546 4 View
I'm working with HEI-OC1 cells of the inner ear. Recently I'm seeing these round particles in the flask (picture attached), but cells' morphology and growth seems normal. However, there are...
26 June 2024 8,717 7 View
I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....
24 June 2024 9,717 0 View
Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...
17 June 2024 3,434 5 View
Or even data that prohibit cartilage harvesting from ear in that age
14 June 2024 5,600 0 View