Can we prepare human islet amyloid polypeptide (IAPP) peptide stock using 5% NH4OH instead of HFIP?

Hi, I am wondering if synthetic human islet amyloid polypeptide (IAPP) can be prepared just like beta-amyloid using NH4OH instead of using HFIP. Concerns: 1- I am not allowed to use...

04 May 2018 3,805 1 View

Fluoresc peak interpretation?

Hi, I could see a peak around ~330 nm for the water sample (MilliQ). This looks strange and I am unable to find the problem. The excitation was 295 nm. We washed the cuvette throughly, but still...

08 September 2017 1,490 8 View

How to generate a coarse grained mapping models for an unknown compound?

04 May 2017 1,923 4 View

What are the possible solution to constrain the C-terminal degradation of the rotamase enzyme?

Dear members, I am trying to express and purify a mouse-derived enzyme (rotamase A) having 7 X His-TEV cleavage site. The protein overexpression at 37C (0.8M IPTG induction for 3 hrs) shows a...

11 December 2016 6,655 3 View

Is 2uL 2-mercaptoethanol is enough for lymphoma and melanoma cell culture?

03 April 2016 4,383 9 View

MiniPrep protocol yields high plasmid concentration for E. coli strains ?

02 March 2016 3,432 8 View

How to monitor the total time required for MD simulation in GPU run (Gromacs) using qsub in PBS cluster?

Dear RG members, I am running MD simulation in GROMACS (GPU) in cluster using qsub. I would like to know when my program will finish which I can not monitor from the GUI. Could somebody help in...

02 March 2016 3,684 0 View

How to calculate number of cells/ml from OD600 values in B. Substilis and S. epidermis ?

01 February 2016 2,472 11 View

How can I generate a small lipid bilayer system in Gromacs ?

01 February 2016 7,303 5 View

Can we grow Lactobacillus acidophilus using LB media instead of recommended MRS media ?

31 December 2015 445 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View