Can we prepare human islet amyloid polypeptide (IAPP) peptide stock using 5% NH4OH instead of HFIP?

Hi, I am wondering if synthetic human islet amyloid polypeptide (IAPP) can be prepared just like beta-amyloid using NH4OH instead of using HFIP. Concerns: 1- I am not allowed to use...

04 May 2018 3,805 1 View

Fluoresc peak interpretation?

Hi, I could see a peak around ~330 nm for the water sample (MilliQ). This looks strange and I am unable to find the problem. The excitation was 295 nm. We washed the cuvette throughly, but still...

08 September 2017 1,490 8 View

What are the possible solution to constrain the C-terminal degradation of the rotamase enzyme?

Dear members, I am trying to express and purify a mouse-derived enzyme (rotamase A) having 7 X His-TEV cleavage site. The protein overexpression at 37C (0.8M IPTG induction for 3 hrs) shows a...

11 December 2016 6,655 3 View

Is 2uL 2-mercaptoethanol is enough for lymphoma and melanoma cell culture?

03 April 2016 4,383 9 View

MiniPrep protocol yields high plasmid concentration for E. coli strains ?

02 March 2016 3,432 8 View

How to monitor the total time required for MD simulation in GPU run (Gromacs) using qsub in PBS cluster?

Dear RG members, I am running MD simulation in GROMACS (GPU) in cluster using qsub. I would like to know when my program will finish which I can not monitor from the GUI. Could somebody help in...

02 March 2016 3,684 0 View

How to calculate number of cells/ml from OD600 values in B. Substilis and S. epidermis ?

01 February 2016 2,472 11 View

How can I generate a small lipid bilayer system in Gromacs ?

01 February 2016 7,303 5 View

Can we grow Lactobacillus acidophilus using LB media instead of recommended MRS media ?

31 December 2015 445 5 View

How does the body control the synthesis of higher antimicrobial peptide concentration?

Dear RG members, I would like to know how the body control the antimicrobial peptide (AMP) production during infection in a concentration dependent pathway. It is well known that, many of the AMPs...

11 December 2015 781 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View