How to find strain energy release rate GI, GII, GIII in Abaqus?

I modeled a simple crack in abaqus. I get J-integral as output as well as components of stress intensity factor KI, KII, KIII. However, I do not know how to get GI, GII and GIII. I searched in...

04 January 2021 1,783 2 View

How to analysis gears contact in Asnsys static structural with frictionless contact type?

I model and simulate the contact between two gear. I applied torque on one of the gears and I fix the other using fix displacement in Ansys. Automatic contact detects the contact between gears...

20 December 2019 869 3 View

What are the modes of heat transfer?

Heat transfer principle

01 February 2019 4,672 1 View

How can measured the low velocity of high air temperature?

experimental device for low velocities of gases

24 January 2019 2,137 1 View

What was the correlation obtained for average Nusselt number for free convection from incnined surfaces?

free convection from inclined surfaces

31 December 2018 6,890 0 View

What is the biot number in heat transfer? and where it used ?

what is the biot number in heat transfer? and where it used ?

31 December 2018 4,717 0 View

What is meaning of the Gratez number?

heat transfer

31 December 2018 1,314 2 View

Does any of the scholars here happen to know about a journal good for vocabulary measurement studies?

I am looking for a journal related to the foloowing title: WAT and VKS: Measures of depth but predictors of breadth of vocaulary

12 August 2018 5,492 1 View

Is it possible to upgrade python version of Abaqus?

Even the latest version of Abaqus ran with python 2.7 and it comes with numpy version 1.6. To be able to use latest version of scipy, I need to upgrade numpy. In addition, the package does not...

26 July 2018 3,348 3 View

How to write heat transfer abaqus subroutine to get steady state soultion?

I know that by using the UMATHT subroutine, one can write a heat transfer equation subroutine, but it is for transient analysis. Can I use same subroutine to get steady state solution or I must...

23 December 2017 193 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View