Deemah Dabbagh

4 Questions 5 Answers 0 Followers

Questions related from Deemah Dabbagh

AAV purification protocol using chromatorgraphy columns for serotype 8?

Does anyone have a protocol (that has worked) or can suggest a protocol of AAV8 purification using an ion exchange column or any type of chromatography. I am looking for a procedure that does not...

10 January 2018 9,999 2 View

Regarding AAV vectors,when we lyse the cells by repeated freeze-thaw to extract virus, do these count as AAV vector freeze-thaw exposures?

If I used 3-4 freeze thaw cycles to retrieve my AAV particles from 293T cells. Does this mean that my virus has already been exposed to repeated freezing and thawing, and that I can no longer...

28 September 2017 5,612 2 View

How can I test the sensitivity of CD4 T cells to a particular antigen?

If I want yo test the sensitivity of CD4+ T cells to a particular Ag. Is it correct to treat with antigen and stimulate with anti CD3/CD28 or do I have to use antigen presenting cell?

03 May 2016 4,762 2 View

Can someone help with the tubular structures formed by bunyaviruses during their maturation?

Are these structures part of the Golgi membrane itself? or are they independent viral structures?

13 September 2015 9,774 1 View