RefSeq problem in PrimerBLAST?

23 January 2019 0 3K Report

I am working on sheep mRNA, and designing primers using NCBI gene and PrimerBLAST. For some genes, there are mRNAs under "RefSeq maintained independently of annonated genome" and also under "RefSeq of annotated genome". Which one gives better primers, and should I even use the former one if I don't get specific primers from annotated genome primers.

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Science method

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