does 2% fbs induce autophagy?
i am studying the effect of certain chemical on my cells, where in grow them in 2% serum (to prevent proliferation due to serum), however, my control cells (with 2% serum and no chemical) itself show increased lc3b II expression.
Serum and potassium deprivation-induced neuronal death on the primary culture of rat cerebellar granule neurons is being widely used as an in vitro model of neurodegeneration and neuronal apoptosis. Activation of the autophagosomal–lysosomal compartment plays an important role in neuronal death induced by serum and potassium deprivation in cultured cerebellar granule cells.
yes, serum content affect the study of autophagy. when serum content reduce the cells undergoes stress and induce autophagy.
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1. Jeffery Ho, Jun Yu, Sunny H. Wong, Lin Zhang, Xiaodong Liu, Wai T. Wong, Czarina C. H. Leung, Gordon Choi, Maggie H. T. Wang, Tony Gin, Matthew T. V. Chan & William K. K. Wu (2016) Autophagy in sepsis: Degradation into exhaustion?, Autophagy, 12:7, 1073-1082, DOI: 10.1080/15548627.2016.1179410
2. Li Zhu, Huaizhou Wang, Yu Wu, Zhengwen He, Yanghua Qin, and Qian Shen, “The Autophagy Level Is Increased in the Synovial Tissues of Patients with Active Rheumatoid Arthritis and Is Correlated with Disease Severity,” Mediators of Inflammation, vol. 2017, Article ID 7623145, 9 pages, 2017. doi:10.1155/2017/7623145
3. Yinfang Wu , Weimin Fan ,Dongsheng Huang , , Xiaodong Sun .Possible intermediary role of autophagy in serum albumin decrease-associated cardiovascular events among patients with coronary heart disease; International Journal of Cardiology 250 (2018) 64.
4. Li Sun, Song Zhang, Chengyuan Yu, Zhenwei Pan. Hydrogen sulfide reduces serum triglyceride by activating liver autophagy via the AMPK-mTOR pathway. Am J Physiol Endocrinol Metab 309: E925–E935, 2015.
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