9 Questions 13 Answers 0 Followers
Questions related from Muhammad Imran
I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...
16 February 2021 3,943 4 View
i need to understand on how to neutralize the tca in supernatant after protein precipitation. I am interested in the deproteinized part and not the pellet proteins. Can an equal volume of Tris pH...
21 January 2021 6,260 2 View
I have tried almost all methods (varying cell no., time, speed of rotation, etc) for generation of compact jAR spheroids to be used for in vitro implantation assays, however, i am unable to get...
03 March 2020 10,071 2 View
my control and experimental cells, both show a strong LC3II on blots, however the experimental set shows an increase, and the LC3I band for both arises only on long exposures and that too is...
19 February 2018 2,618 3 View
probing for pAMPK: blocking - 5% bsa primary - 2% bsa o/n secondary - 2% bsa washings with 0.1% tbst my queries are: should i be blocking with nfdm? and secondary also with nfdm? and for total...
31 October 2017 639 3 View
increased s phase in teh cell cycle should also be complemented with an increased g2m phase? in context to cell cycle analysis using PI ?
16 September 2017 594 3 View
I wish to perform PI staining on ishikawa cells for cell cycle analysis, would like to know the optimum amount of RNAse A to be added here for 1 million cells?
21 September 2015 5,074 4 View
what is preferable, whether autoclaving or filter sterilizing pbs for cell culture use?
26 November 2014 6,572 2 View
And what role does urea play in LB? Importance of protease inhibitor in LB?
25 June 2014 6,542 1 View