Muhammad Imran

5 Questions 11 Answers 0 Followers

Questions related from Muhammad Imran

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

16 February 2021 3,916 4 View

What are the alternate methods for generation of jAR spheroids?

I have tried almost all methods (varying cell no., time, speed of rotation, etc) for generation of compact jAR spheroids to be used for in vitro implantation assays, however, i am unable to get...

03 March 2020 10,052 2 View

PAMPK western blot?

probing for pAMPK: blocking - 5% bsa primary - 2% bsa o/n secondary - 2% bsa washings with 0.1% tbst my queries are: should i be blocking with nfdm? and secondary also with nfdm? and for total...

31 October 2017 630 3 View

Cell proliferation using flow cytometry indicates an increased population in the s phase, does it also mean the population in g2m should also increas?

increased s phase in teh cell cycle should also be complemented with an increased g2m phase? in context to cell cycle analysis using PI ?

16 September 2017 583 3 View

Should I filter sterilize or autoclave 1x PBS for cell culture?

what is preferable, whether autoclaving or filter sterilizing pbs for cell culture use?

26 November 2014 6,558 2 View