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I am Ayah Soufan, a 3rd-year Ph.D. researcher at Strathclyde University interested in designing systems to help scholars conduct literature reviews. If you are a Master's student in Computer...

13 April 2023 9,454 1 View

How to handle large seurat objects (5GB+) in R?

I have integrated 11 seurat objects that I need to merge before downstream analysis, I combined the first 4 (2.6GB total) and saved them as an rds object just fine but every time I combine the...

22 November 2022 1,785 3 View

How to map Affymetrix probes from microarray data into gene symbols or IDs for Clariom_S_Human annotation?

I have microarray data from Affymetrix Clariom S Assay, Human chips, I read the .cel files but when I do the RMA normalization I get the following error: " Error in getCdfInfo(object) : Could...

19 September 2022 5,570 1 View

How to perform a Differentially methylated regions analysis (DMRs/DMC) using GenomicRationSet?

I want to find the differentially methylated regions between two groups of samples, I have a dataset containing the beta values for each sample mapped to each gene and the corresponding grouping...

24 July 2022 8,869 0 View

Have you ever produced a literature review? Can you help me make the experience better?

I’m doing a Ph.D. to design tools to make it easier for people to review the literature. Can you help me make it better? Please answer this questionnaire which will take around 10 minutes only,...

13 September 2021 6,443 4 View

How to avoid having multiple differenet Tm peaks in the qrt-PCR Melt Curve when having 3 different genes ?

Hi, I have 3 genes (1st is my Gene of interest and two house keeping genes) each gene will have a forward primer , a reverse primer and a different Tm for each primer. 1-When doing the cDNA...

03 May 2021 3,739 4 View

Hi all ,What is the best protocol and reagents to extract microRNA from platelets for qrt-PCR ?

am looking for a protocol to extract microRNA from platelets where I can use for qrt-PCR any recommendations please?

22 April 2021 4,733 3 View

Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

24 December 2020 6,874 3 View

How to ensure right PBMC's count?

hello there! I am isolating PBMC's from heparinized blood using density gradient method and after having the pelette we count cells using a hemocytometer by taking 5 ul cells with 10 ul trypan...

19 October 2020 4,341 19 View

How to measure cell suspension concentration or wight?

hello there! I'm going to use M-per protein extraction kit for PBMC'S , the protocol recommended to add 1 ml of the reagent for each 100 mg or 100 μL cell suspension so how could we measure cell...

02 October 2020 5,621 2 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (E-ISSN 1901-9726, P-ISSN : 0900-9671) a legitima?

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...

01 August 2024 629 3 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

Alliance Tony-Mayeko

Hello.

My answer has been recorded to the Qualtrics Survey site (https://strathsci.qualtrics.com/jfe/form/SV_a4tKVnGledsUyq2?jfefe=new)

Lee Clawson

Seems like the survey form already expired. And....A rather sad example of user interfaces in itself. However, if you're trying to learn more about user interfaces I strongly recommend looking outside of the Computer Science field.