Recovery chamber rodents?

More Masa Čater's questions See All

How can I find out how to reach a person by email to send them full texts of my articles?

How can I find out how to reach a person who wants full texts of one of my several articles listed on ResearchGate, so I may email the requestors one of my several articles they are requesting?

27 February 2024 1,957 0 View

How to avoid the droplets coalescence in microfludic system?

I've been using Span 80 to stabilize droplets in my microfluidic system. Initially, 3wt% was effective, but I'm now facing coalescence issues in the output chamber inside the chip. I've tried 2wt%...

20 February 2024 4,549 0 View

Can I use automatic baseline in qPCR if I want to compare results between plates?

I ran the same qPCR test (SYBR Green) on a lot of samples that didn't fit on one plate (over 20 plates all together) and want to compare all of them to the same standard curve that was included in...

18 November 2021 3,417 0 View

What is the best probe for measuring chlorophyll a and phycocyanin in the field?

I'm looking into AlgaeTorch (bbe Moldaenke), matrixFlu VIS/microFlu/nanoFlu (TriOS) and Cyclops-7F (TurnerDesigns). I couldn't find any application notes on any of those. Does anybody have some...

09 November 2021 2,527 3 View

Can evaporation of PCR reactions lead to contamination in the cycler?

The whole volumes of my PCR reactions (10 uL) evaporated during the cycling (honest mistake, I know why it happened and how to fix it - wrong PCR caps used). My question is, could this leave some...

23 January 2021 4,104 4 View

Has anybody used ELISA kits for determination concentration of folic acid in cell lysates?

I would like to treat cells with folic acid and then determine the concentration of it in cell lysates with enzyme-linked immunosorbent assay kit (ELISA). Because of that I am looking for the...

13 September 2016 9,876 1 View

Does anyone have a protocol for isolation and culture of mouse primary alveolar macrophages?

I am working on lung in vivo infections, but I now need to do some in vitro experiments with primary alveolar macrophages. i am using mice with B6 background. I have established a protocol where I...

19 August 2015 4,939 8 View

What is the best way to obtain an idea of magnetic nanoparticles' aggregation in wax (or similar materials)?

There are several studies in which they disperse magnetic nanoparticles in wax, talc, etc. in order to decrease their aggregation, but they generally do not show clear evidence of the...

20 February 2014 6,913 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View