In the analysis of micropollutants from environmental samples, surrogates are often used to monitor recoveries in extraction and cleanup. These surrogates are often isotope labelled analytes. For analysis in GC/LC-MS, internal standards are used to compensate matrix effect and instrumental bias, and to be strictly, they are also isotope labelled analytes. For a given compound, only one kind of labelled compound is commercially available normally. For example, only d15-triphenyl phosphate is offered as labelled triphenyl phosphate commercially. My question is, if I spike d15-triphenyl phosphate to soil sample to do recovery test, how do I quantify d15-triphenyl phosphate in GC/LC-MS? What internal standard should I use to compensate matrix effect in GC/LC-MS analysis of d15-triphenyl phosphate? In many papers, they only mention that they spike d15-triphenyl phosphate to sample and get a recovery. I am confused that whether they use calibration with internal standard to quantify d15-triphenyl phosphate. Anyone has an idea on this? Thank you in advance!
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